Electric blast drying oven to test trace elements in finished medicine
The old cough and asthma tablets, compound Banxia tablets, Weitongning tablets, boxing tablets and ginseng were determined by electric blast drying oven.
Ca, Mg, Fe, Zn, K, Na, 7 kinds of Chinese patent medicines, such as Qijiangtang capsule, Xia Sangju granule and American ginseng
The content of Mn, Cu, Cr, Co, Sr, Ni, Cd and Pb elements. Detailed study of the type of digestive juice (mixed acid),
The effect of HNO3 and HClO4 ratio, digestive juice volume and digestion time on the determination results in mixed acid. Result table
Ming: The content of Ca, Mg, Fe, K and Na in the selected 7 drugs is generally rich; drugs for treating diabetes
The content of Mn in Shenqi Jiangtang Capsule is rich, while that in Stomach Tablets is rich in Sr, Mn and Cu.
Key words: electric blast drying oven; proprietary Chinese medicine; trace elements In recent years, trace elements have great biological effects, physiological functions and practical value of clinical diagnosis and treatment.
It has caused people's interest in researching inorganic elements, especially trace elements in proprietary Chinese medicines. Modern research shows that in
In the process of drug efficacy, the synergistic effect of trace elements can not be ignored, and the proper amount of trace elements can not only promote
Into the growth and development of the body, but also can improve the body's immune function, reducing human susceptibility to disease.
The determination of trace elements can provide basic data for clarifying the mechanism of action, modification and innovation of new drugs.
Can provide a basis for the identification and improvement of Chinese herbal medicines. The electric blast drying oven has high sensitivity and good selectivity.
High precision and fast analysis. In this paper, the elderly cough and asthma tablets were first determined by electric blast drying oven.
Compound Banxia Tablets, Weitongning Tablets, Boxing Tablets, Shenqi Jiangtang Capsule, Xia Sangju Granules and American American Ginseng Tablets
The contents of Ca, Mg, Fe, Zn, K, Na, Mn, Cu, Cr, Co, Sr, Ni, Cd and Pb in Chinese patent medicines.
The type of digestive juice (mixed acid), the ratio of HNO3 and HClO4 in the mixed acid solution, the volume of the digestive juice and
The effect of digestion time on the results of the assay. This study can analyze the relationship between the efficacy of the above-mentioned proprietary Chinese medicines and trace elements.
To clarify its mechanism of action and to modify and innovate new drugs to provide certain information.
1 Experimental part
1.1 Instrument and working conditions Atomic absorption spectrophotometer, 14 element hollow cathode lamps.
1.2 reagents and standard solutions
Standard solutions of 14 elements (Ca, Mg, Fe, Zn, K, Na, Mn, Cu, Cr, Co, Sr, Ni, Cd, Pb)
It was purchased from the National Standards Research Center (the concentration of the stock solution was 100 μg/mL except for Cr of 1 000 μg/mL). HNO3, HClO4, H2SO4, HCl, H2O2. The experimental Water is ultrapure water.
1.3 Sample source and treatment The 7 kinds of Chinese patent medicines used in the experiment, such as Guchuan Tablet, Compound Banxia Tablet, Weitongning Tablet, Boxing Tablet, Shenqi Jiangtang Capsule,
Xia Sangju granules and American ginseng lozenges are commercially available.
Preparation of the sample solution: Take an appropriate amount of the drug (the pellet is pre-ground with a mortar and the capsule is filled with the capsule)
(Shanghai Ring Competition test production), drying at 100 ° C for 1 h, cooling and placed in a desiccator. Accurately weigh about 1g of medicine
In a small 100 mL beaker, add 20 mL of HNO3-HClO4 (3:1) mixed acid, heat and digest for 40 min, quantitative transfer and determination
Allow up to 100mL. Take the supernatant and measure (if necessary, appropriate dilution).
2 Results and discussion
2.1 Optimization of sample processing conditions
2.1.1 Selection of mixed acid species To find a suitable digestive system, several HNO3-HClO4, HNO3-HCl, HNO3-H2SO4 and HNO3-H2O2 were studied.
The effect of the digestive system on the results of the assay. The experimental results show that it is suitable to use HNO3-HClO4 mixed acid system as digestive solution.
2.1.2
The effect of HNO3 and >HClO4 volume ratio in HNO3-HClO4 mixed acid on the content of Cu, Fe, Zn, Mn and Ni in the boxing tablets
As an example, the volume ratio of HNO3 and HClO4 in the mixed acid digest was 1:1, 3:1, 4:1, 5:1, 7:1, respectively.
9:1 ​​impact. Visible, HNO3
When the volume ratio of -HClO4 is 3:1, the digestion effect is good.
2.1.3 The influence of digestion time is still determined by the determination of Cu, Fe, Zn, Mn and Ni. The use of HNO3-HClO4 mixed acid with a volume ratio of 3:1 is used as a
The system was fixed in a volume of 15 mL, and the effect of digestion time on the measurement results was investigated. The results show that the 40min elimination
The best time to achieve the best digestion.
2.1.4 Influence of digestive juice volume The 3:1 HNO3-HClO4 mixed acid system was used as the digestive juice with a fixed digestion time of 40 min and a sample mass of 1.0 g.
The effect of mixed acid volume on the determination results of 8 , 12 , 16 , 20 , 24mL respectively (still in Cu , Fe , Zn , Mn in the boxing tablets )
And the content of Ni is determined as an example). It can be seen that the mixed acid volume is 20 mL to obtain satisfactory measurement results.
2.2 Standard curve and method accuracy test Diluted the stock solution of 14 elements into a series of standard solutions with appropriate concentration, using the six standard standard curve method, as listed in Table 1.
Instrument conditions were measured. The results show that the working curves of the 14 elements are all straight lines, and the correlation coefficients are all greater than 0.997.
The linear relationship of the working curve is good. In order to further investigate the accuracy of the method, the recovery rate of spiked tablets was taken as an example.
Test (To ensure accuracy, the spiked recovery test was done in parallel, 3 times in parallel, and then averaged).
2.3 Sensitivity and detection limits The sensitivity and detection limits of the method were calculated according to the literature.
2.4 Determination of 14 trace elements in the sample Accurately weigh 1.0 g of sample and treat the sample according to the sample processing method in Section 1.3. Dissolve the sample according to the working conditions of the instruments listed in the table
The liquid was measured. Place
2.5 Discussion From the measurement results, it can be seen that the content of five elements of Ca, Mg, Fe, K and Na in the selected seven Chinese patent medicines is generally rich.
However, with the different effects of variety and function, the content of trace elements in each drug is also very different. Such as treating diabetes
It is not accidental that the Mn content in the drug is high. Animal experiments have shown that when the body is deficient in Mn, its insulin B cells decrease.
Pancreatic hypoplasia, impaired glucose tolerance, and a corresponding decrease in glucose utilization. Stomach pain tablets contain higher Sr, Mn and
Cu element, etc. At the same time, harmful Cd and Pb are also present in certain drugs. These harmful elements may
Some links from raw materials, solvents or processing processes should be of concern.
references
[ 1 ] DONG Shun2fu , ZHU Zhi2guo ( Dong Shunfu , Zhu Zhiguo ) . Spectroscopy and Spectral Analysis
(Spectroscopy and Spectral Analysis), 2002, 22 (3) : 478.
[ 2 ] FU Ke2jun (Foke Army) . Trace Elments in Human Body . Beijing : Chinese Medicine Science and Technology Press (Beijing: China Medical Science and Technology Press), 1995. 82.
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