The microbiology laboratory is a basic laboratory in the field of biology. For a complete microbiology laboratory, which instruments do we need to configure? Shanghai Tiancheng Medical Flow provides the following references for your microbiological experimental instrument configuration:
(1) Ultra-clean bench microbial culture is carried out in a specific medium for sterile culture, then sterile culture will inevitably require a clean bench to provide a sterile working environment. The purpose of the ultra-clean workbench is the aseptic processing of microbial inoculation and handling.
(2) There are many types of incubator incubators, which serve to provide a suitable environment for the growth of microorganisms. There are many types of incubators, including biochemical incubators, mold incubators, and anaerobic incubators. Specifically, the differences are as follows:
a. The biochemical incubator can only control the temperature. It can be used as a plate culture for general bacteria.
b. The mold incubator can control temperature and humidity. Can be used as a mold for cultivation.
c. The CO2 incubator is suitable for the cultivation of anaerobic microorganisms such as bifidobacteria.
(3) Balance balance is used to accurately weigh various reagents. Electronic balances are commonly used in laboratories, and electronic balances have different levels depending on the accuracy.
(4) A microbial homogenizer is used to extract bacteria from a solid sample. The microbial detection sample prepared by the microbial homogenizer has the characteristics of no pollution, no damage, no temperature rise, no need for sterilization treatment, no need for washing utensils, and the like, and is a convenient instrument for use in microbial experiments.
(5) Colony counter colony counter can assist the operator in counting the number of colonies. Accurately obtain the number of colonies by zooming in, taking photos, counting, etc. Some high-performance colony counters can also be connected to a computer for automatic counting.
(6) The microwave oven/electric furnace is used for rapid heating of the solution, and the microorganism solid medium is heated and dissolved.
(7) Most of the experimental materials, reagents and culture media used in microbiology of autoclave should be strictly sterilized. Sterilizers are available in different sizes, some are manual and some are fully automatic. Users need to choose according to their own needs.
(8) Pipette liquid meter is used to accurately measure various types of liquid. Common liquid measuring devices include measuring cylinders, pipettes, micro-pumps, graduated test tubes, and beakers.
(9) Refrigerator The refrigerator is an essential instrument for storing reagents and samples in the laboratory. Some reagents used in microbiology experiments are required to be stored at 4 degrees, and some requirements are stored at minus 20 degrees. The experimenter must see the storage conditions of the reagents and store them at the appropriate temperature. Specifically, items stored at different temperatures are as follows:
a. 4 ° C is suitable for storing certain solutions, reagents, drugs, etc.
b. -20 °C is suitable for certain reagents, drugs, antibiotics, etc.
c. -80 °C is suitable for the preservation of certain long-term cryopreserved samples, E. coli strains, competent state, and the like.
(10) The reagents and sample microorganisms involved in the microbiological experiment of the biosafety cabinet are somewhat toxic and are harmful to the operator. In order to prevent the spread of harmful aerosols and aerosols, biosafety cabinets can be used to provide safety protection for cross-contamination and environmental protection between operators, samples and samples.
(11) The shaker shaker is a commonly used instrument in the laboratory. During the microbial experiment operation, the liquid medium needs to be oscillated at a specific temperature when cultivating the bacteria.
(12) Pure water device The pure water device includes a distilled water machine and a pure water machine. The price of the distilled water is cheap, but it needs to be guarded during the water making process; the pure water machine is more expensive, but it is convenient to use and can store a certain amount of pure water. There are also different levels of pure water use. In the experiment, reagents are prepared and pure water is required to prepare the medium.
(13) Since the biological Microscope has a small volume of microorganisms, it is necessary to use a biological microscope for observation. Biological microscopes are used for the observation of the structure, morphology, etc. of microorganisms and tiny objects.
(14) The freeze dryer is mainly suitable for drying bacteria, microorganisms, yeast, and the like. It is used for drying and preserving products that are easy to dehydrate. After adding water, it can restore the characteristics of raw materials without affecting its biological activity. By freeze-drying, materials such as bacteria become dry, so that no chemical changes occur.
(15) The spectrophotometer spectrophotometer is used in the microbiological test to determine the concentration of the microbial suspension, and the appropriate culture time can be correctly selected. The concentration of the bacterial solution is generally measured at a wavelength of 600 nm.
(16) Constant temperature drying oven The constant temperature drying oven is used for drying the articles after sterilization and washing. The oven has different temperature control ranges and the user can choose according to the experimental needs. For example, some plastic appliances can only be dried in an oven at 42-45 ° C. Generally, glassware can be dried at 60 ° C.
(17) Constant temperature water bath The water bath is a temperature control device. The temperature control of the water bath is relatively fast and sufficient for the sample. Some microbial reactions require a water bath at 37 degrees, 42 degrees, and 56 degrees, so a constant temperature water bath can provide the required temperature.
(18) The pH meter is used to accurately measure the pH when configuring the reagent to ensure the accuracy of the configured solution. It is sometimes necessary to measure the pH of the sample solution using a pH meter.
(19) Centrifuges are used to collect microbial cells and other sediments. The centrifuge has freezing and room temperature. Some samples are less stable at room temperature and require a low temperature environment depending on the type of sample.
(20) Liquid nitrogen tank The liquid nitrogen tank stores liquid nitrogen, which can be used for long-term preservation of various microorganisms such as bacteria, yeast, mold and large fungi.
The above is the basic instrument configuration of the microbiology laboratory. Other consumables are needed when setting up the laboratory, such as alcohol lamp, test tube rack, triangle bottle, measuring cylinder, glass test tube, sterile straw, cool dry rack, scissors, tweezers, absorbent cotton. , gauze, test tube basket, sterile sampling and weighing bags, inoculating rings, filters, etc.
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