1. The injection system liquid chromatograph generally uses a diaphragm injection injector or a high pressure injection chamber to complete the injection operation, and the injection volume is constant. This is beneficial for improving the repeatability of the analytical sample.
2. Separation system The system includes a column, a connecting tube, a thermostat, and the like. The column is generally 10 to 50 cm in length (when two are used together, a connecting tube can be added between the two), and the inner diameter is 2 to 5 mm. It is made of "high-quality stainless steel or thick-walled glass tube or titanium alloy. The housing contains a stationary phase with a particle size of 5-10 μm (consisting of matrix and fixative). The matrix in the stationary phase consists of a resin with high mechanical strength or silica gel, which are inert (such as the basic silicic acid gene on the surface of silica gel). Has been removed), porosity (pore size up to 1000?) and large specific surface area, plus the surface is mechanically coated (as in the preparation of stationary phase in gas chromatography), or chemically coupled to various genes (such as An organic compound of a phosphate group, a quaternary amino group, a methylol group, a phenyl group, an amino group or an alkyl group of various lengths of a carbon chain, or the like. Therefore, such a substance having a fixed relative structure is excellent in selectivity. After pea agglutinin (PSA) is coupled to the surface of porous silica gel, a glycoprotein in fibroblasts can be isolated. In addition, the stationary phase-based plasmid is small, and the column bed can easily reach a uniform and dense state. It is easy to reduce the eddy diffusion effect. The matrix particle size is small, the micropores are shallow, and the sample has short mass transfer in the microporous region. These are beneficial for reducing the band width and improving the resolution. According to the column effect theory analysis, the matrix particle size is small, the tray The larger the theoretical number N, this further proves that the small particle size will increase the resolution. Furthermore, the thermostat thermostat can adjust the temperature from room temperature to 60C, improving the mass transfer rate and shortening the analysis time. , can increase the efficiency of the column.
3. Infusion system
The system consists of a high pressure pump, a mobile phase reservoir and a gradiometer. The general pressure of the high pressure pump is l. 47～4.4X10Pa, the flow rate is adjustable and stable. When the high-pressure mobile phase passes through the column, the diffusion effect of the sample in the column can be reduced, and the moving speed in the column can be accelerated, which improves the resolution and recovers the sample. It is advantageous to maintain the biological activity of the sample and the like. Mobile phase reservoirs and gradiometers allow the mobile phase to vary with the nature of the stationary phase and sample, including changing the polarity of the eluent, ionic strength, pH, or switching to competitive inhibitors or denaturants. This allows efficient separation of various substances (even if there is only one group difference or isomer).Lateral Filing Cabinets are the most welcomed Filing Drawers used in offices. They are available in 2/3/4 Drawer Cabinets . Various sizes of the Filing Cabinets offer a great deal of flexibility. The elegant product line of the Storage Filing Cabinets, featuring practical and design-like details of the Metal Cabinet With Drawers, provides a stylish framework within which you can create the perfect organized workplace.
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